Secondarily, an increase in noise level is a indication of mechanical deterioration or malfunction. 4. The BSC is not a substitute for good microbiological practices and does not automatically provide you with protection from potentially hazardous materials or automatically prevent contamination of the experiment and materials. Protective goggles should transmit less than 4% of 400 nm wavelength light; 6. E. Dry hypochlorites, liquid bleach, and any other strong oxidizing material, must NOT be autoclaved. The potential pathogenic or infectious organisms subject to BSL-2 standards pose a moderate . Also available in Lab Safety Catalog, page 461. These BSC's rely on the building exhaust system for proper operation. j. Wash and mop adjacent area and spill area with appropriate disinfectant - detergent solution. A 1:10 dilution (5,000 ppm) of bleach has a biocidal effect on M. tuberculosis, S. aureus, other vegetative bacteria, and HIV after 10-20 minutes. 4. In compliance with the Workplace Hazard Assessment - OSHA standard CFR 1910.132. Detailed descriptions of containment practices and biosafety levels can be found in the CDC-NIH Guidelines Biosafety in Microbiological and Biomedical Laboratories (BMBL). Biosafety Level 2 (BSL 2) is similar to Biosafety Level 1 and is suitable for work in clinical, diagnostic, teaching,research or production facilities involving agents of moderate potential hazard to personnel and the environment. Containers used to hold material while being autoclaved are described below. These markings only appear when the tape has been exposed for a few minutes to normal autoclave decontamination temperatures. 3. Nucleic acid containing thymine dimers does not replicate properly and lethal mutations are often produced. Minimize the amount of equipment and supplies; overloading the working zone with equipment and supplies may compromise the effectiveness of your BSC. f. Pour an appropriate disinfectant solution (1:10 dilution of household bleach) around the spill and allow it to flow into the spill. Packaged according to 42 CFR Part 72.3. More effective in alkaline than acid solutions. B. Recombinant DNA molecules contained in an organism or in a viral genome shall be shipped under the applicable regulations of the governmental agencies listed above. BSL-3 risk group contains biological agents that usually cause serious disease (human, animal or plant) or that can result in serious economic consequences. Know the Class and Type of biological safety cabinet (BSC) you are about to use prior to using it. For maximum protection to the operator during blending or mixing of infectious materials, the following practices should be observed. A heat-sealed flexible plastic film enclosure for a grinder or blender can be used, but it must be opened in a biological safety cabinet. 38/No. The need for such a test is based on belief that workers should not be exposed to unnecessary noise. Must withstand leakage of contents, shocks, pressure changes, etc. Biosafety Level 2 (BSL 2) is similar to Biosafety Level 1 and is suitable for work in clinical, diagnostic, teaching,research or production facilities involving agents of moderate potential hazard to personnel and the environment. Symptoms develop 3 to 12 hours after exposure and usually disappear in a few days. J. Infect Control. 2. Standard Number 49. 3. 6. Cover the pan with a suitable cover and autoclave according to standard directions. 2. There are four biosafety levels (BSL). Because of potential hazards associated with the shipment of biohazardous materials, state and federal regulations control shipping practices. Polypropylene is a durable, inexpensive plastic resin which is commonly used contain material during autoclaving. Patient specimens that would be expected to contain an etiologic agent should be shipped according to 42 CFR Part 72.3 (Etiologic Agent). 6. Classification of Etiologic Agents on the Basis of Hazard. When the notice of receipt is not received within 5 days following anticipated delivery the sender must notify CDC. This class includes pathogens which require special conditions for containment, usually at Biosafety Level 3. Whenever possible, use test tube trays with a solid bottom and sides deep enough to hold all spillage from broken tubes. 10. Discard the closed container into a red bag-lined biohazard box. 11. C. All autoclaves should be certified for operating efficiency by the frequent use of biological indicator controls. 5. They are heavy and may have sharp edges and corners. The Area Safety Office has several audiovisual programs for instruction. Use of steam sterilizers (autoclaves) should include validation of decontamination effectiveness. 1. 1. Centrifuge models should be chosen which have a sealed rotor, or sealed buckets, or with at least a guard bowl and gasketed cover. A. ATCC determines the biosafety level of a product or material based on our risk assessment as guided by the current edition ofBiosafety in Microbiological and Biomedical Laboratories (BMBL), U.S. Department of Health and Human Services. See Lab Safety Supply catalog. These guidelines were developed to insure the safety of people who work with ventilated cabinets. 6. WescodyneTM (AMSCO) works well in intermediate temperature water baths. Do not, under any circumstances, put laboratory waste outside the building. 2. The laboratory is in a separate building or in an isolated and restricted zone of the building. They must be used for biological agents ed with toxic chemicals and radionuclides. 1. Before use, the plastic sharps containers must be put in the metal stand designed for the container. Clinical or other laboratories handling human blood or tissue should be aware of possible biohazardous aerosols produced by microhaematocrit centrifuges, autoanalyzer stirrers, microtonometers, FACS's, etc. 1. MR 21-1/1990E, 1990. B. It is essential that anti-foam spraybe added to the overflow flask since air bubbling through the disinfectant- immersed sparger may produce foam that could shut off the vacuum if allowed to clog the filter. When you have completed this lesson, you will be able to recognize characteristics of the four biological safety levels. If you are using a vacuum flask system in your BSC, put a vacuum protection device, a filter and flask, in line to protect the vacuum system. d. Wait 60 minutes to allow dissipation of spill-created aerosols by the room ventilation air changes. Clean and disinfect the spill pan at least four times per year or as necessary. 10. This purges the air from the internal BSC cabinetry. Allow the BSC to operate an additional 10 minutes, then turn the blower motor off. A sink and eyewash are readily available. Do not clean the spill pan when a BSC blower is operating. Class II (Laminar Flow) Biohazard Cabinetry, National Sanitation Foundation, Ann Arbor, Michigan, Revised May, 1992. The building exhaust system must be equipped with a bag-in/bag-out HEPA filter caisson to provide environmental protection. All solid, autoclave-decontaminated materials must be discarded into the biohazard box. The nature of the infectious agent, toxin, or other biological hazard, including: The BSL assigned to a project determines the controls required to conduct the work safely: Every biological laboratory, regardless of biosafety level, follows standard microbiological practices. Please enable scripts and reload this page. A HEPA filter should be placed between the centrifuge inner chamber and the vacuum pump when containment is needed. (CDC) 93-8395. All infectious or toxic materials, and all contaminated equipment or apparatus should be decontaminated before being washed and stored, or discarded. 2. Access to the laboratory is restricted when work is being conducted. The biological indicator is removed from a load after it has been autoclaved. 6. 55, No. Keep fresh disinfectant available at the proper dilution. Recognizing the Biosafety Levels This Quick Learn Lesson will take approximately 15 minutes to complete. This test is performed to determine the face velocity of inflow air (supply air) through the work access opening, and the calculated exhaust flow volume. Shower with a germicidal soap. Transportation of potentially pathogenic/oncogenic fluid cultures or toxins and viable, powdered biohazardous materials should be transported, incubated, and stored in easily handled, non-breakable, leak-proof pans, trays, pails, carboy holders, or other secondary containers large enough to contain all the fluid or powder in case of leakage or breakage of the primary container. 2. 3. Combine iodine with non-ionic detergent. 6. When work has been completed, disinfect the exterior surfaces of potentially contaminated materials and supplies with appropriate disinfectant before removing them from the BSC. Materials which the shipper reasonably believes does not contain an etiologic agent should ship according to 42 CFR Part 72.2. Recovering paper towels can only be accomplished by decontamination and disassembly of the cabinet by an authorized service technician. The sign, DO NOT EAT, SMOKE OR DRINK indicates that eating, drinking, smoking, handling of contact lenses, and applying cosmetics are not permitted in the posted work area because there is reasonable likelihood of exposure to hazardous chemicals, radioactive materials or potentially infectious materials. (Parts for mounting unit are in catalog, page 455. D. Special precautions should be taken to prevent accidental removal of material from an autoclave before it has been sterilized or decontaminated. The correct technical name is "biological safety cabinet" or the acronym "BSC". Where tubes of different material are provided (e.g., celluloid, polypropylene, stainless steel), make sure that you are using the tube closure that was designed specifically for the tube being used. C. Sterilization the biological indicator and associated documentation is required by regulation. 4.2.4 Biosafety Level-4 Laboratories Page 18 . LockA locked padlock Several primary containers in one secondary container if total contents of primary containers doesn't exceed 50 ml. Chemical indicators should be positioned near the center of each load, and toward the bottom front of the autoclave. D. UTILIZATION OF QUART PLASTIC CONTAINERS (TWO, EIGHT GALLON). Be careful when removing interior work surfaces. The supply HEPA filter provides particle-free vertical laminar airflow over the work surface, which protects the product from contamination. The logbook should be available for inspection by various agencies, authorities, and the NAA Safety Office. Every effort should be made to limit the number of books and journals kept in biohazardous areas. Install all necessary items for your experiment in the BSC at this time. This establishs guidelines for the effective use of steam autoclaves for the decontamination of cultures and other potentially pathogenic materials and protects personnel and the environment from these potentially infectious materials and waste. For further information on shipping etiologic agents, please contact: 1. Existing ventilated cabinets and ancillary equipment, such as thimble exhaust ducting, gas, electric and vacuum systems, must be "CLEARED FOR MAINTENANCE" by the Area Engineering & Safety , or local supporting engineering department prior to disassembly. Add disinfectant slowly to outer margin of spill and allow it to flow in. Class I - Open Face Biological Safety Cabinets: DESCRIPTION: A partial barrier biological safety (BSC) with unfiltered, non-recirculated inward airflow away from the operator and over the work surface. 9. Each rotor should be accompanied by its own log book indicating the number of hours run at top or de-rated speeds. The heart of a ventilated cabinet is the high efficiency particulate air (HEPA) filter. The filter and vacuum flask should be decontaminated by autoclaving if they have been in contact with potentially biohazardous material. A common error people make with ventilated cabinets is mistaking a chemical fume hood (hood) or clean air bench (CAB) for a specialized cabinet called a biological safety cabinet (BSC). 4. BSL-3 laboratories are designed to be easily decontaminated. The HEPA filter removes particulate material, such as dust, fungal spores, bacteria, and viruses from the internal cabinet air. ) or https:// means youve safely connected to the .gov website. Needles must not be recapped using both hands. Materials that are known to contain, or could contain, etiologic agents. All exhaust air is discharged to the outside after HEPA filtration. You can select the "Continue Account Application" button below if you need to complete your application. The containers must be placed into an approved biohazard box, never directly into a red bag. Some of these audiovisuals are listed below: A. LGC Standards is the exclusive distributor of ATCC products for your location. Effectiveness reduced by organic material. These procedures must be performed in biosafety cabinet class II, and containment level 3 . Effective June 24, 1994, Published in Federal Register, July 5, 1994 (59 FR 34472) Amendment Effective July 28, 1994, Federal Register, August 5, 1994 (59 FR 40170) Ann Arbor, Michigan, February 1, 1993. The aerosol/fluid trap consists of two vacuum flasks, preferably plastic, (size dependent on amount of fluid that may accidentally be aspirated out of the collection flask), thick walled plastic tubing (to prevent tubing collapse), rubber stoppers, a filter (prevents unwanted potentially biohazardous fluid and aerosols from entering vacuum systems), and a ceramic sparger (ceramic fish tank bubbler) immersed in disinfectant. into a autoclave bag lined deep autoclave pan. An asterisk (*) indicates select agents. Floor cleaning procedures which minimize the generation of environmental aerosols should be used. ATCC does not distribute Biosafety Level 4 (BSL-4) products. 1. Dry powders are difficult to decontaminate, particularly with liquid disinfectants. b. Organize your material so that dirty "contaminated" items are not passed over (cross contaminate) clean items. Do not substitute glass, metal or plastic jars, bottles or cans. Applying cosmetics, including hand lotion and lip balm, are prohibited in laboratories. c. It is not necessary to flame items. Share sensitive information only on official, secure websites. Associating an account to your profile allows you to place an order on atcc.org. Make sure the view screen window is adjusted (if adjustable) to the proper height (usually 10 inches). They must be discarded into a biohazard box. A few examples of odor control additives are: any absorbent kitty litter, "Odo-Clave", "Decon Decap" - Thomas Scientific. After this time, the lamp may not produce enough germicidal radiation to effectively kill bacteria, even though it appears to be functioning properly. Class II, Type B2 BSC's typically have one internal supply air HEPA filter. E. After the ventilated cabinet is moved, it must be recertified according to applicable performance standard. There are four biosafety levels (BSL). Turn off the ultraviolet (UV) lamp, turn on the fluorescent light, and inspect the air intake grilles for obstructions and foreign materials. High speed rotor heads are prone to metal fatigue. 6. If a problem is found, promptly notify your area supervisor who will call facilities or maintenance. In addition, there is monitoring for occupationally acquired infections and staff training, as appropriate. The assignment of a biosafety level to a particular work process is made through a protocol driven risk assessment. These devices release considerable aerosols during operation. C. BIOHAZARD CABINET CONTAINMENT EQUIPMENT. 1. 5. However, an accident resulting in breakage of a centrifuge tube or bottle can result in a massive exposure. H. FOREIGN QUARANTINE (42 CFR PART 71.54 and 72.3). A minimum average inflow velocity of 100 fpm is maintained through the work access opening. C. Performance tests/certifications must be conducted only by a qualified contractor. 3. Stable in storage if kept cool and tightly covered. 5. Use dilution usually non-irritating to skin but prolonged skin or eye contact should be avoided. Use safety blenders designed to prevent leakage from the rotor bearing at the bottom of the bowl. Although these show poor activity against bacterial spores, they are recommended for general use in concentrations of 70 to 150 ppm. An updated In general, the larger the load, the longer the decontamination time. All inoculated petri plates or other inoculated solid media should be transported in leak-proof containers. 2. This can return a vapor or gas contaminant back to the "clean" work space and back into the laboratory until equilibrium is reached. Screening Framework Guidance for Providers of Synthetic Double-Stranded DNA, This page last reviewed: September 25, 2019, U.S. Department of health & Human Services, Science Safety Security Finding the Balance Together, U.S. Department of Health and Human Service. OSHA Instruction CPL 2-2.44C, March 6, 1992. A secondary function of the test is to clean the lamps so they operate more efficiently. Work from "clean" to "dirty" areas. Federal Register, vol. Laboratory waste must never be discarded directly into the general trash or into red bags. Additionally, host organisms and viruses will be shipped as etiologic agents if they contain recombinant DNA. Low pressure mercury vapor lamps usually supplied with biological safety cabinets emit germicidal radiation at a wavelength of 254 nanometers for about nine months. Some are active against lipophilic viruses. Cold cathode, low ozone lamps, are preferred. Avoid unnecessary use of syringes and needles. Enter your email to sign up. Remember that the BSC air curtain is delicate and can only provide protection from contamination as long as it is not disrupted. Ultraviolet (UV) lighting, when requested by the purchaser, shall be installed so that it does not reduce the required performance of the BSC. A. The Biosafety Protocol should list all rooms that are being used for your study. The "Type" of BSC dictates the configuration of the cabinet. Please turn on JavaScript and try again. 3. 1. 2. 1. You've successfully associated your account with your Profile. This will also meet the waste disposal requirements of the State of Maryland, AAMI, AHA, CDC, and JCAHO. Swab the site of injection with an appropriate antiseptic before and after injection of an animal. HEPA filtered downflow air comes from a common exhaust plenum that is a mixture of downflow supply air and laboratory supply air. (OSHA standard 1910.95)shall be posted with the hearing protection required sign. B. HAZARDOUS OPERATIONS (TWO PERSON RULE) AND THE SECURED LABORATORY. Stop other work while the equipment is operating. 4. The Industrial Environment - Its Evaluation and Control, U.S. Dept. BSL-2 laboratories must also have access to equipment that can decontaminate laboratory waste, including an incinerator, an autoclave, and/or another method of decontamination, depending on the biological risk assessment. 6. Low solubility in water unless combined with detergent. If decanting is necessary, the outer rim should be wiped with a disinfectant. When possible, reusable syringes with attached needles should be placed in a stainless steel pan separate from that holding materials to be discarded after autoclaving. In labs at biosafety level 2 and above, signs on the outside of the door should indicate biosafety level . c. Allow disinfectant to act for 20 to 30 minutes before cleaning up with more paper towels. Biosafety Level 2 (BSL 2): builds upon BSL-1. Do not breathe, leave the room immediately, and close the door. In no instance should such a use of disinfectant solution be considered a sterilization procedure. Particular care needs to be exercised around UV gel transilluminators, they produce considerable radiation. 39, no. When laboratory vacuum is used to manipulate biohazardous materials, a suitable trap must be employed to insure that building vacuum pump and compressed air lines (they are often linked together in the building mechanical room) do not become contaminated. A sink must be available for hand washing. (NIH) 75-790. The inside of trunnion cups should be inspected for rough walls caused by erosion or adhering matter, and glass pieces should be carefully removed from the rubber cushion. The laboratories incorporate all BSL-3 features, as well as, additional safety features. Agent Type: Bacteria . 9. Each biohazard container used for disposal of laboratory waste must contain a red bag liner or marked bag at least 2 mil thick or equivalent. Biosafety Level -1 (BSL-1) Requirements Standard microbiological practices Safety practices Safety equipment Uses Organisms 2. 1. Your chair should be adjusted to a comfortable height that promotes proper posture. a. 7. B. Class II BSC's must be used for all activities involving low or moderate risk biohazardous agents, etiologic agents, oncogenic viruses, and recombinant DNA experiments requiring Biosafety Level 1, 2, and 3 containment. Rubber O-rings and tube closures must be examined for deterioration and be kept lubricated with material recommended by the manufacturer (high temperature silicone vacuum grease). CLASSIFICATION OF ETIOLOGIC AGENTS ON THE BASIS OF HAZARD. Autoclaved pasteur pipettes, needles and syringes are then discarded into the biohazard box. E. UTILIZATION OF THE BIOHAZARD STEP-ON CONTAINERS OR BAG HOLDERS. E. At times there may be requirements for special cabinets to protect workers from airborne transmission of biohazardous agents or materials. If you work in a lab that is designated a BSL-2, the microbes there pose moderate hazards to laboratorians and the environment. 5. You should work at least six inches back from the front of the air intake grille. b. Operate blending, cell-disruption and grinding equipment only in a biological safety cabinet. Biosafety is the application of safety precautions that reduce a users risk of exposure to a potentially infectious material and limit contamination of the work environment and the community. In addition, the laboratory space is designed to also prevent contamination of other spaces. Pasteur pipettes, needles and syringes, and similar "sharps" contaminated with cultures should be placed into a, polypropylene autoclave bag-lined, stainless steel or polypropylene pan with cover containing enough disinfectant solution, such as Wescodyne (diluted 1:10), to cover the pipettes, and then autoclaved. Cleaning is the responsibility of the personnel in charge of the laboratory. Refer to Section III -- Recombinant DNA Guidelines. The chemical fume hood, or "hood", is specifically designed for use with potentially hazardous chemicals and is not generally used with potentially biohazardous materials. The autoclave log book should have at least the following information entered; 2. ; table tops shall be wiped down with an appropriate disinfectant; contaminated glassware and equipment shall be sterilized or contained in covered pans. The exhaust HEPA filter provides particle-free BSC exhaust air. 39, no. 3. Failure to have an appropriate import permit may result in confiscation of a shipment at a port of entry. H. Disinfect all work surfaces before, during and after BSC use. The sign shall be taken down immediately after the source of danger is removed. The Class II, Type B2 exhaust air is typically HEPA filtered through an ancillary HEPA filter installed upstream in a dedicated building exhaust system. This test is performed to determine the integrity of supply and exhaust HEPA filters, filter housings, and filter mounting frames. Stainless steel containers are durable and come in a variety of sizes and shapes. Refrigerators, freezers and dry ice chests should be checked, cleaned out, and defrosted periodically to remove any ampules, tubes, etc., containing biohazardous materials that may have broken during storage. Under certain conditions of radiation intensity, exposure time, humidity, and temperature, ultraviolet radiation at approximately 254 nanometers will cause eventual death of microorganisms. Wash your hands thoroughly before and after working in your BSC. Higher temperatures and shorter times may be used for heat-resistant materials. When planned work involves chemical carcinogens or solvents, it is necessary to evaluate the quantities to be used to determine the amount that might be entrained in the BSC air stream during an accident. 5. Effective against Gram-positive organisms, bacteriostatic in high dilutions. Ultraviolet light's greatest effectiveness is against actively growing bacteria. When discarded sharps reach the fill level designated on the sharps container (at the constriction), screw on the cap, remove the closed container from the metal stand. Type B2 BSC's must be "hard connected" to the building exhaust system. D. DEFINITIONS (42 CFR Part 72 - Interstate Shipment of Etiologic Agents, 1980). An Occupational Health Program exists for medical surveillance of laboratory workers. The CDC regulates importation of all etiologic agents and hosts and vectors of human disease. Cabinets may be required for shaking machines, animal waste containers, and centrifuges. * Non autoclaveable supplies can be used, but will be discarded after incident. Access to BSL-4 laboratories is carefully controlled and requires significant training. Examples of BSL-1 organisms are: Agrobacterium radiobacter, Aspergillus niger, Bacillus thuringiensis, Escherichia coli strain K12, Lactobacillus acidophilus, Micrococcus leuteus, Neurospora crassa, Pseudomonas fluorescens, Serratia marcescens. Engineering controls, including facility construction and design elements in the laboratory space; Administrative controls, including specific access, training, and occupational medicine policies; and. All exposed UV installations in lighting fixtures and safety cabinets shall be turned on only when no personnel are in the area. C. Contact your Facilities Management office for instructions on how your ventilated cabinet will be physically moved. IES Lighting Handbook, 5th Ed, Illuminating Engineering Society, New York, NY, 1972. Operating ultrasonic disintegrators, vortex mixers or other high energy equipment. Should be nonparticulate and absorb entire contents of primary container(s). Flood the top work surface tray, and, if a Class II cabinet, the drain pan below the work surface, with a disinfectant and allow to stand 20 minutes. This list is dynamic and undergoes periodic updating as new information is learned about currently-classified agents and as novel agents emerge. Are you sure you don't want to sign up to get news from ATCC? Restaurant supply companies are a good source for these pans. (Handle sharps containers with caution, they are puncture resistant, not puncture proof). Three (3) levels of risk have been established. I. ORGANISMS & VECTORS - USDA IMPORTS (9 CFR PART 122.2) (Reserved). 14. Secure .gov websites use HTTPS The interior lab has a phone and emergency contact numbers available. There is no recirculated air in this BSC; 7. Refrigerators, freezers, and cold rooms in laboratories where work with biohazardous materials is conducted should not be used for storage of lunches or other food supplies. A broken glass box with a red bag liner is not an acceptable substitute for a red bag-lined biohazard box. Polypropylene plastic pans with 6-12 inch sides are favored over polyethylene and polystyrene because polypropylene can withstand autoclaving without melting. 1. Broken glass boxes are not acceptable substitutes for biohazard boxes. The HEPA filter is positioned within the cabinets' ducting. 5. After moving a BSC from one location to another. Where waste containment is mandatory, stainless steel containers may be the container of choice because it is durable. These are classified as partial containment biological safety cabinets (BSC's, see figure 2(d). 9. All laminar flow BSC's must be performance tested ("certified") annually by qualified personnel. 9. Description: Salmonella enterica is one of two Salmonella species and is a member of the Enterobacteriaceae family. S-4 Supplement. Prolonged contact deteriorates rubber. 8. The descriptions of biosafety levels (BSL) 1 4 parallel those in the NIH Guidelines for research involving recombinant DNA. Biohazardous agents that are under BSL3 are indigenous, exotic and may cause serious or lethal disease through respiratory transmission. You may be trying to access this site from a secured browser on the server. Personnel should wear proper personal protective equipment, ie. The regulations for shipment of etiologic agents and recombinant DNA materials state that: "No person may knowingly transport or cause to be transported in interstate traffic, directly or indirectly, any material, including but not limited to, diagnostic specimens and biological products, which such person reasonably believes may contain an etiologic agent, unless such material is packaged to withstand leakage of contents, shocks, pressure changes, and other conditions incident to ordinary handling in transportation.". You can also start a new application by selecting the "Start a new account application" below to establish another account with ATCC. Box 649Mill Creek RoadMarietta, OH 45750 (800) 848-3080, Germfree Germfree Laboratories, Inc. 7435 N.W. Louvered, wall mounted UV equipment may be left on continuously. All plastic sharps containers that have been autoclaved may have "sharps" protruding through the side. 5. A specific facility operations manual is prepared, reviewed by the Biosafety Officer, and adopted. 4.2.3 Biosafety Level-3 Laboratories Page 15 . Examine the spill pan beneath the work surface. 235, December 6, 1991, p. 64175-64182. Use of an alcohol pleget around the stopper and needle when removing a syringe and needle from a rubber stoppered vaccine bottle. Tape indicators are adhesive backed paper tape with heat sensitive, chemical indicator markings. PPE(lab coats, gloves, eye protection) are worn. Acceptable as general use disinfectants to control vegetative bacteria and non-lipid containing viruses. Its use must be limited and controlled because of its toxic properties. See Lab Safety Supply Catalog starting at Page 455. If your autoclave is equipped to operate at 132oC (270oF), you may be able to reduce processing time if biological indicator spores placed in the center of the material to be decontaminated are killed during shorter autoclaving times. F. Public Health Service Statement on Management of Occupational Exposure to Human Immunodeficiency Virus, Including Considerations Regarding Zidovudine Postexposure Use, Morbidity & Mortality Weekly Report, vol. A. Hands must be washed throughout the day, at intervals dictated by the nature of the work. 2. A 0.5 percent sodium hypochlorite solution (1 to 10 dilution of liquid laundry bleach) is recommended for decontamination of HBV, HIV, and cleanup of biohazardous spills.

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